Viral Membrane Proteins Laboratory

Group leader
José L Nieva
Phone
+34 94 601 3353/3429
Address

Biofisika Institute (CSIC-UPV/EHU)
Science Park of the UPV/EHU
Barrio Sarriena s/n. 48940 Leioa, Bizkaia (Spain)

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Research goal

Our objective is to determine the molecular mechanism by which membrane glycoproteins of some viruses (HIV, Ebola) induce the fusion of the cell and viral membranes. Prediction tools have been developed to detect the domains that are inserted into the target membrane. In parallel, we are studying the mechanism of cell membrane permeabilisation, induced by certain viral products (viroporins) during infection (this work on viroporins is in collaboration with L. Carrasco, CBM, Madrid).

Group members
Postdoctoral Fellow
Research Technician
University Professor
Postdoctoral Fellow
Publications

Rujas E, Cui H, Burnie J, Aschner CB, Zhao T, Insausti S, Muthuraman K, Semesi A, Ophel J, Nieva JL, Seaman MS, Guzzo C, Treanor B, Julien JP.

Engineering pan-HIV-1 neutralization potency through multispecific antibody avidity. Proc Natl Acad Sci U S A. 2022 Jan 25;119(4):e2112887119. PMID: 35064083; PMCID: PMC8795538.
doi: 10.1073/pnas.2112887119

Rujas E, Leaman DP, Insausti S, Carravilla P, García-Porras M, Largo E, Morillo I, Sánchez-Eugenia R, Zhang L, Cui H, Iloro I, Elortza F, Julien JP, Eggeling C, Zwick MB, Caaveiro JMM, Nieva JL.

Focal accumulation of aromaticity at the CDRH3 loop mitigates 4E10 polyreactivity without altering its HIV neutralization profile. iScience. 2021 Aug 17;24(9):102987. PMID: 34505005; PMCID: PMC8413895.
doi: 10.1016/j.isci.2021.102987

Caillat C, Guilligay D, Torralba J, Friedrich N, Nieva JL, Trkola A, Chipot CJ, Dehez FL, Weissenhorn W.

Structure of HIV-1 gp41 with its membrane anchors targeted by neutralizing antibodies. Elife. 2021 Apr 19;10:e65005. PMID: 33871352; PMCID: PMC8084527.
doi: 10.7554/eLife.65005

de la Arada I, Torralba J, Tascón I, Colom A, Ubarretxena-Belandia I, Arrondo JLR, Apellániz B, Nieva JL.

Conformational plasticity underlies membrane fusion induced by an HIV sequence juxtaposed to the lipid envelope. Sci Rep. 2021 Jan 14;11(1):1278. PMID: 33446748; PMCID: PMC7809034.
doi:10.1038/s41598-020-80156-w

Largo E, Queralt-Martín M, Carravilla P, Nieva JL, Alcaraz A.

Single-molecule conformational dynamics of viroporin ion channels regulated by lipid-protein interactions. Bioelectrochemistry. 2021 Feb;137:107641. Epub 2020 Aug 24. PMID: 32889489; PMCID: PMC7444495.
doi: 10.1016/j.bioelechem.2020.107641

Rujas E, Insausti S, Leaman DP, Carravilla P, González-Resines S, Monceaux V, Sánchez-Eugenia R, García-Porras M, Iloro I, Zhang L, Elortza F, Julien JP, Saéz-Cirión A, Zwick MB, Eggeling C, Ojida A, Domene C, Caaveiro JMM, Nieva JL.

Affinity for the Interface Underpins Potency of Antibodies Operating In Membrane Environments. Cell Rep. 2020 Aug 18;32(7):108037. PMID: 32814041; PMCID: PMC7861656.
doi: 10.1016/j.celrep.2020.108037

Torralba J, de la Arada I, Carravilla P, Insausti S, Rujas E, Largo E, Eggeling C, Arrondo JLR, Apellániz B, Nieva JL.

Cholesterol Constrains the Antigenic Configuration of the Membrane-Proximal Neutralizing HIV-1 Epitope. ACS Infect Dis. 2020 Aug 14;6(8):2155-2168. Epub 2020 Jul 8. PMID: 32584020.
doi: 10.1021/acsinfecdis.0c00243

Carravilla P, Nieva JL, Eggeling C.

Fluorescence Microscopy of the HIV-1 Envelope. Viruses. 2020 Mar 21;12(3):348. PMID: 32245254; PMCID: PMC7150788.
doi: 10.3390/v12030348

Carravilla P, Darré L, Oar-Arteta IR, Vesga AG, Rujas E, de Las Heras-Martínez G, Domene C, Nieva JL, Requejo-Isidro J.

The Bilayer Collective Properties Govern the Interaction of an HIV-1 Antibody with the Viral Membrane. Biophys J. 2020 Jan 7;118(1):44-56. Epub 2019 Nov 14. PMID: 31787208; PMCID: PMC6950647.
doi: 10.1016/j.bpj.2019.11.005

Carravilla P, Chojnacki J, Rujas E, Insausti S, Largo E, Waithe D, Apellaniz B, Sicard T, Julien JP, Eggeling C, Nieva JL.

Molecular recognition of the native HIV-1 MPER revealed by STED microscopy of single virions. Nat Commun. 2019 Jan 8;10(1):78. PMID: 30622256; PMCID: PMC6325134.
doi: 10.1038/s41467-018-07962-9