Single-Molecule Nanopore Biophysics
Group leader
David Rodriguez-Larrea
Email
Phone
+34 946 01 8002
Address
Biofisika Institute (CSIC-UPV/EHU)
Science Park of the UPV/EHU
Barrio Sarriena s/n. 48940 Leioa, Bizkaia (Spain)
Research goal
Our research is focused on understanding protein translocation through membrane pores and on the biotechnological applications of such processes.
Group members
Publications
Alfaro JA, Bohländer P, Dai M, Filius M, Howard CJ, van Kooten XF, Ohayon S, Pomorski A, Schmid S, Aksimentiev A, Anslyn EV, Bedran G, Cao C, Chinappi M, Coyaud E, Dekker C, Dittmar G, Drachman N, Eelkema R, Goodlett D, Hentz S, Kalathiya U, Kelleher NL, Kelly RT, Kelman Z, Kim SH, Kuster B, Rodriguez-Larrea D, Lindsay S, Maglia G, Marcotte EM, Marino JP, Masselon C, Mayer M, Samaras P, Sarthak K, Sepiashvili L, Stein D, Wanunu M, Wilhelm M, Yin P, Meller A, Joo C.
The emerging landscape of single-molecule protein sequencing technologies.
Nat Methods. 2021 Jun;18(6):604-617.
DOI: 10.1038/s41592-021-01143-1
Rodriguez-Larrea D.
Single-aminoacid discrimination in proteins with homogeneous nanopore sensors and neural networks.
Biosens Bioelectron. 2021 May 15;180:113108.
DOI: 10.1016/j.bios.2021.113108
Feng J, Martin-Baniandres P, Booth MJ, Veggiani G, Howarth M, Bayley H, Rodriguez-Larrea D.
Transmembrane protein rotaxanes reveal kinetic traps in the refolding of translocated substrates.
Commun Biol. 2020 Apr 3;3(1):159.
DOI: 10.1038/s42003-020-0840-5
Rosen CB, Bayley H, Rodriguez-Larrea D
Free-energy landscapes of membrane co-translocational protein unfolding.
Commun Biol. 2020 Apr 3;3(1):160.
DOI: 10.1038/s42003-020-0841-4
Rodríguez J, Learte-Aymamí S, Mosquera J, Celaya G, Rodríguez-Larrea D, Vázquez ME, Mascareñas JL
DNA-binding miniproteins based on zinc fingers. Assessment of the interaction using nanopores.
Chem Sci. 2018 Apr 10;9(17):4118-4123.
DOI: 10.1039/c7sc05441f