Integrative Structural and Functional Characterization of Macromolecular Assemblies Laboratory
Our laboratory is interested in the structural and functional characterization of macromolecular assemblies, with special emphasis on the large protein complex that regulates transport between the nucleus and the cytoplasm in eukaryotic cells: the nuclear pore complex (NPC). Apart from its main role as mediator of nucleocytoplasmic transport, the NPC is also a key platform for the regulation of many essential nuclear processes, such as chromatin organization, gene expression, or DNA repair. It is thus not surprising that defects affecting the NPC function are directly related to severe human diseases, like several types of cancers and neurological disorders. To understand how the NPC performs its roles we use a combination of approaches, including biochemical, proteomic, cell and structural biology methods, that are finally combined into integrative structure-function maps to reveal the basic molecular mechanisms underlying the NPC function.
Akey CW, Singh D, Ouch C, Echeverria I, Nudelman I, Varberg JM, Yu Z, Fang F, Shi Y, Wang J, Salzberg D, Song K, Xu C, Gumbart JC, Suslov S, Unruh J, Jaspersen SL, Chait BT, Sali A, Fernandez-Martinez J*, Ludtke SJ*, Villa E*, Rout MP*.
Ricardo-Lax I, Luna JM, Thao TTN, Le Pen J, Yu Y, Hoffmann HH, Schneider WM, Razooky BS, Fernandez-Martinez J, Schmidt F, Weisblum Y, Trüeb BS, Berenguer Veiga I, Schmied K, Ebert N, Michailidis E, Peace A, Sánchez-Rivera FJ, Lowe SW, Rout MP, Hatziioannou T, Bieniasz PD, Poirier JT, MacDonald MR, Thiel V, Rice CM.
Hakhverdyan Z, Molloy KR, Subbotin RI, Fernandez-Martinez J*, Chait BT*, Rout MP*.
Fernandez-Martinez J, Rout MP.
Hakhverdyan Z, Molloy KR, Keegan S, Herricks T, Lepore DM, Munson M, Subbotin RI, Fenyo D, Aitchison JD, Fernandez-Martinez J*, Chait BT*, Rout MP*
Kim SJ*, Fernandez-Martinez J*, Nudelman I*, Shi Y*, Zhang W*, Raveh B, Herricks T, Slaughter BD, Hogan J, Upla P, Chemmama IE, Pellarin R, Echeverria I, Shivaraju M, Chaudhury AS, Wang J, Williams R, Unruh JR, Greenberg CH, Jacobs EY, Yu Z, de la Cruz MJ, Mironska R, Stokes DL, Aitchison JD, Jarrold MF, Gerton JL, Ludtke SJ, Akey CW, Chait BT, Sali1 A, Rout MP.