Euro-Bioimaging Node - Advanced Light Microscopy Node Bilbao (ALM@BIO)

Research goal

The Bilbao Advanced Light Microscopy Node Candidate (ALM@BIO) is a joint initiative of the imaging platforms of the Instituto Biofisika (CSIC, UPV/EHU) and Achucarro Basque Center for Neuroscience, both located in the Science Park of the University of the Basque Country (UPV/EHU) in the metropolitan area of Bilbao. The Node Candidate is focused on quantitative imaging of cellular events such as molecular interactions and biophysical studies such as lipid-mediated protein interactions or lipid-order imaging of model membranes and cells; and on the neurobiology of neuron-glia interactions.

Specialties and expertise of the Node Candidate

ALM@BIO provides assistance during the whole experimentation process: experiment design, training, image analysis and storage.
The Node Candidate offers experimental configurations that are not available commercially and home-made application-tailored software with a particular emphasis on membrane-related measurements and neurobioimaging, such as:

 - Spatio-temporal study of intracellular molecular interactions (FRET-FLIM)

 - Ligand-receptor binding (FCS)

 - Lipid-mediated protein interactions (scanning FCS)

 - Intact-cell imaging of lipid concentration at cellular membranes

 - Super resolution imaging of primary cells and slices of the CNS

 - Live imaging of genetically encoded calcium indicators ​

Offered Technologies​

 - Fluorescence correlation spectroscopy (FCS)

 - Fluorescence cross-correlation spectroscopy (FCCS)

 - Fluorescence-lifetime imaging microscopy (FLIM)

 - Fluorescence resonance energy transfer (FRET)

 - Stimulated emission depletion microscopy (STED)

Instrument highlights

Special features of the instruments at the Bilbao Node Candidate are:

 - Tailor-made set-up for scanning FCCS for the study of lipid-mediated protein interactions

 - Live cell imaging capabilities: CO2 chamber and resonant scanner

 - In-house developed analysis software tailored to each application

 - Stimulated emission depletion microscopy (Gated CW-STED)​