Euro-Bioimaging Node - Advanced Light Microscopy Node Bilbao (ALM@BIO)
The Bilbao Advanced Light Microscopy Node Candidate (ALM@BIO) is a joint initiative of the imaging platforms of the Instituto Biofisika (CSIC, UPV/EHU) and Achucarro Basque Center for Neuroscience, both located in the Science Park of the University of the Basque Country (UPV/EHU) in the metropolitan area of Bilbao. The Node Candidate is focused on quantitative imaging of cellular events such as molecular interactions and biophysical studies such as lipid-mediated protein interactions or lipid-order imaging of model membranes and cells; and on the neurobiology of neuron-glia interactions.
Specialties and expertise of the Node Candidate
ALM@BIO provides assistance during the whole experimentation process: experiment design, training, image analysis and storage.
The Node Candidate offers experimental configurations that are not available commercially and home-made application-tailored software with a particular emphasis on membrane-related measurements and neurobioimaging, such as:
- Spatio-temporal study of intracellular molecular interactions (FRET-FLIM)
- Ligand-receptor binding (FCS)
- Lipid-mediated protein interactions (scanning FCS)
- Intact-cell imaging of lipid concentration at cellular membranes
- Super resolution imaging of primary cells and slices of the CNS
- Live imaging of genetically encoded calcium indicators
Offered Technologies
- Fluorescence correlation spectroscopy (FCS)
- Fluorescence cross-correlation spectroscopy (FCCS)
- Fluorescence-lifetime imaging microscopy (FLIM)
- Fluorescence resonance energy transfer (FRET)
- Stimulated emission depletion microscopy (STED)
Instrument highlights
Special features of the instruments at the Bilbao Node Candidate are:
- Tailor-made set-up for scanning FCCS for the study of lipid-mediated protein interactions
- Live cell imaging capabilities: CO2 chamber and resonant scanner
- In-house developed analysis software tailored to each application
- Stimulated emission depletion microscopy (Gated CW-STED)